Recombinant Protein Production Service
 
Reliable Recombinant Proteins by an IVD manufacturer

Features

Pilot expression screening
Construct design with 3D insights
Buffer optimization

Pilot construct design for recombinant proteins and antibodies

Affinity Tag Options for pilot construct design
Affinity tag Capture ligand
His-Tag Nickel or Cobalt
GST Glutathione
DDDDK-tag Anti-DDDDK antibody
HA-tag Anti-HA antibody
Myc-tag Anti-Myc antibody
BirA-tag (Site-specific biotinylation) Streptavidin
E-tag Anti-E-tag antibody
In silico 3D model evaluation to assess native‑like structural integrity prior to expression.
Antibody format options for chimeric antibody design
Antibody format Light chain Typical purification ligand
Human IgG1 κ / λ Protein A or Protein G
Human IgG2 κ / λ
Human IgG3 κ / λ
Human IgG4 (S228P) κ / λ
Mouse IgG1 κ
Mouse IgG2a κ
Mouse IgG2b κ
Rabbit IgG κ / λ
Human IgA κ / λ Lectin
Mouse IgM κ Lectin
Chicken IgY λ Subclass‑specific antibody
Purification strategies are selected based on antibody isotype and subclass.

Buffer optimization

Custom buffer solutions leveraging our expertise in protein stabilization.

Approach

1. Thermal stability tests by DSF and narrow down appropriate composition.
2. UPLC-SEC before and after stress by freeze-thaw cycles or 37℃.
3. DLS analysis.

Screening method Monitored Characteristics
DSF (Tm) Thermal stability
SEC-HPLC Honogeneity & Aggregation propensity
DLS (Tagg) Aggregation propensity
DLS (KD/B22) Colloidal stability

Expression systems

Host Types of proteins

RPDS_Expression_systems_img-01-1

CHO cells

 IgG, Non-IgG

RPDS_Expression_systems_img-02-1

HEK 293 cells

Baculovirus

Non-IgG

E.coli
Host Types of proteins

RPDS_Expression_systems_img-01-1

CHO cells

 IgG, Non-IgG

RPDS_Expression_systems_img-02-1

HEK 293 cells

Baculovirus

Non-IgG

E.coli

Protein Characterization

  • Concentration (based on Absorbance at 280 nm) 
  • SDS-PAGE
  • SEC-UPLC (Size-Exclusion Chromatography using UPLC) 
  • DSF, SLS, DSL including Tagg and B22 (e.g., UNCLE) 
  • Stability test (37℃ & freeze-thaw) 
  • Endotoxin Measurement (LAL assay) 
  • Mycoplasma Detection (PCR amplification) 

About MBL

MBL was founded in 1969 as the first manufacturer of antibodies in Japan. Using technology built on advances in immunology and molecular biology, MBL offers clinical diagnostics and research reagents designed for analysis of proteins and/or genes associated primarily with autoimmune disorders, cancer, gynecological diseases, and infectious diseases. In recent years MBL has also been actively developing biomarker reagents and companion diagnostics, taking advantage of our strength in immunological and gene detection techniques.

→ About US